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26 March 2021: Animal Study

Salvianic Acid A Regulates High-Glucose-Treated Endothelial Progenitor Cell Dysfunction via the AKT/Endothelial Nitric Oxide Synthase (eNOS) Pathway

Yanhua Guan 12ABCDEF , Xu Wang 13ABCDEF*

DOI: 10.12659/MSM.928153

Med Sci Monit 2021; 27:e928153

Figure 4 Effects of combined salvianic acid A (SAA) (100 μmol/L) and phosphoinositide 3-kinase (PI3K) inhibitor on the expressions of Akt/endothelial nitric oxide synthase (eNOS) pathway-related proteins, cell viability, lactated dehydrogenase (LDH) and nitric oxide (NO) production in high-glucose (HG)-treated EPCs. (A, B) the expression of Akt/eNOS pathway-related proteins was measured by western blotting. *** P<0.001 compared to the control group. ### P<0.001 compared to the HG group. @@@ P<0.001 compared to the HG+SAA group. (C) The ratio of phosphorylated (p)-Akt/Akt. *** P<0.001 compared to the control group. ### P<0.001 compared to the HG group. @@@ P<0.001 compared to the HG+SAA group. (D) The ratio of phosphorylated (p)-eNOS/eNOS. *** P<0.001 compared to the control group. ### P<0.001 compared to the HG group. @@@ P<0.001 compared to the HG+SAA group. (E) Detection of cell viability after pretreatment with SAA and PI3K inhibitor. *** P<0.001 compared to the control group. ## P<0.01 compared to the HG group. @ P<0.05 compared to the HG+SAA group. (F) The levels of LDH after pretreatment with SAA and PI3K inhibitor. *** P<0.001 compared to the control group. ### P<0.001 compared to the HG group. @@ P<0.01 compared to the HG+SAA group. (G) The level of NO production after pretreatment with SAA and PI3K inhibitor. *** P<0.001 compared to the control group. # P<0.05 compared to the HG group. @ P<0.05 compared to the HG+SAA group. The cells were divided into 4 groups: control group (5.5 mmol/L glucose and 25 mmol/L mannitol), HG group (30 mmol/L glucose), HG+SAA group (cells were pretreated with 100 μmol/L SAA for 24 h and then cultured by 30 mmol/L glucose for 24 h) and HG+SAA+LY294002 group (cells were pretreated with 100 μmol/L SAA for 24 h and LY294002 for 1 h and then cultured with 30 mmol/L glucose for 24 h).

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750