09 March 2021>: Lab/In Vitro Research
Gasdermin D Influence Mouse Podocytes Against High-Glucose-Induced Inflammation and Apoptosis via the C-Jun N-Terminal Kinase (JNK) Pathway
Huifang Li 1ABCDE , Kunxiao Zhao 2ADF , Ying Li 1ADFG*DOI: 10.12659/MSM.928411
Med Sci Monit 2021; 27:e928411
Figure 3 Effects of JNK inhibitor on inflammation and apoptosis in mouse podocytes induced by HG. (A, B) The activities of JNK and p-JNK were evaluated via western blotting. (C–E) The activities of Bax, Bcl-2, cleaved caspase-3, IL-1β, IL-6, and TNF-α were evaluated via western blotting. (F–H) The intensity of synaptopodin in mouse podocytes was detected via western blotting and immunofluorescence. NG – normal-glucose; HG – high-glucose; NG+SP – normal-glucose+SP600125; HG+SP – high-glucose+SP600125. Data represent the average of 3 measurements. Mean±SD of 3 experiments is shown. * P<0.05, ** P<0.01 vs NG, # P<0.05, ## P<0.01 vs HG.