25 March 2021: Lab/In Vitro Research
Knockdown of Chitinase 3-Like-1 Inhibits Cell Proliferation, Promotes Apoptosis, and Enhances Effect of Anti-Programmed Death Ligand 1 (PD-L1) in Diffuse Large B Cell Lymphoma Cells
Xiao Yang 1AC* , Dong Fang 2B* , Ming Li 1F , Jiayi Chen 1B , Yuanbo Cheng 1F , Jianming Luo 1DE*DOI: 10.12659/MSM.929431
Med Sci Monit 2021; 27:e929431
Figure 2 Knockdown of CHI3L1 reduces cell proliferation and cell cycle arrest in vitro. (A) Western blot analysis of CHI3L1 protein expression in SU-DHL-4. (B) RT-PCR analysis of CHI3L1 expression in SU-DHL-4. (C) ShRNA-CHI3L1-1 cells, ShRNA-NC cells, and the control cells were seeded into a 96-well plate. Cells were treated with CCK8, and the CCK8 absorbance value was measured 24 h, 48 h, and 72 h after cell seeding. (D) The effects of ShRNA-CHI3L1-1 on SU-DHL-4 cell proliferation were measured by CCK8 assay. (E) Western blot analysis of cyclinD1 and cyclinD2 expression in SU-DHL-4. Data are presented as mean±SD. Results shown here are the representative of 3 independent experiments. ** p<0.01, *** p<0.005 vs Control.