Figure 4 TCONS_00068220 promotes EMT and E-cadherin was downregulated in breast cancer. (A) Western blot assay was used to evaluate the protein expression of EMT markers in response to TCONS_00068220 overexpression in MCF-7 cells or knockdown in MDA-MB-231 cells. (B) E-cadherin level in breast cancer and adjacent normal tissues. (C) Negative correlation between expression levels of TCONS_00068220 and E-cadherin in breast cancer tissues. (D) E-cadherin level in MCF10A and various breast cancer cells. EMT, epithelial-mesenchymal transition. * P<0.05 and ** P<0.01 vs control.