Figure 5 Analysis of spine density in PFC layer V pyramidal neurons (Golgi-Cox staining, Scale bar: 5 μm). (A) Apical dendrites in layer V pyramidal neurons of the PFC, the prelimbic subregions (PrL) were observed. Representative images are shown of high-magnification projections of layer V pyramidal neuron apical tuft dendrites (scale bar: 5 μm). (B–D) The total spine density, mature, immature spine density. Results are presented as mean±SEM (5 dendritic segments from 1 animal. Three rats for each group). *** p<0.001 compared with the Normal group; ## p<0.01, ### p<0.001 compared with the Stress group, ^^^ p<0.001 compared with the Phys+stress group. The figure was created using Adobe Photoshop V2019 and GraphPad Prism version 7.0 software.