10 April 2006
Physiologic microcirculation of the heterotopically transplanted rat liver with portal vein arterialization depending on optimal stent diameter.
Karina Schleimer, Dirk L. Stippel, Hans-U Kasper, Christian Suer, Samir Tawadros, Samir Tawadros, K.Tobias E. BeckurtsMed Sci Monit 2006; 12(4): BR140-145 :: ID: 448895
Abstract
Background: Clinical experience with portal vein arterialization (PVA)in liver transplantation is controversial. One reason for this is the lack of standardized flow regulation.The present experiments aimed to establish flow regulation in the arterialized portal vein for heterotopicauxiliary liver transplantation (HALT), to obtain physiological portal blood flow, and to compare thistechnique with orthotopic liver transplantation. Material/Methods: Lewis rats were divided into 7 groups(n=8 transplantations/group). Group: A I-IV: In HALT, the portal vein was anastomosed to the right renalartery using stents with different diameters (0.2, 0.3, 0.4, 0.5 mm). Afterwards, HALT with PVA usingthe stent diameter that had achieved the most physiological portal blood flow (group B II) was comparedwith orthotopic liver transplantation with porto-portal anastomosis (group B III) and to the sham group(B I). Results: After reperfusion, only the 0.3 mm stent resulted in an average blood flow in the arterializedportal vein in HALT which was within the normal range (1.7+/-0.4 ml/min/g liver weight). The parametersof microcirculation and early graft function were significantly better in group B II than in group BIII (functional sinusoidal density: 335+/-48 vs. 224+/-31/cm, diameter of sinusoids: 6.4+/-0.6 vs. 5.2+/-0.6microm, diameter of postsinusoidal venules: 31.1+/-3.3 vs. 25.5+/-2.0 microm, bile-production: 27+/-8vs. 19+/-5 microl/h/g liver weight). Conclusions: Using an optimal stent diameter in HALT with portalvein arterialization, an adequate flow-regulation can be achieved. Avoiding portal hyper- and hypoperfusion,good results for microcirculation and early graft function can be obtained.
Keywords: Arteriovenous Shunt, Surgical, Liver Circulation - physiology, Liver Transplantation - physiology, Microcirculation - physiology, Portal Vein - surgery, Rats, Inbred Lew, Stents, Transplantation, Heterotopic - physiology, Transplantation, Isogeneic
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
08 Mar 2024 : Clinical Research
Evaluation of Foot Structure in Preschool Children Based on Body MassMed Sci Monit In Press; DOI: 10.12659/MSM.943765
15 Apr 2024 : Laboratory Research
The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in OsteoarthritisMed Sci Monit In Press; DOI: 10.12659/MSM.943738
07 Mar 2024 : Clinical Research
Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...Med Sci Monit In Press; DOI: 10.12659/MSM.943468
08 Mar 2024 : Animal Research
Modification of Experimental Model of Necrotizing Enterocolitis (NEC) in Rat Pups by Single Exposure to Hyp...Med Sci Monit In Press; DOI: 10.12659/MSM.943443
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952