Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

02 March 1998

Effect of antioxidants and of the xanthine oxidase inhibitor on the level of free radicals as well as the antioxidative activity of enzymes in rat skeletal muscles during acute ischemia and reperfusion

Kinga Mikrut, Hanna Krauss, Jacek Kozlik, Paweł Chęciński, Janusz Paluszak, Łukasz Dzieciuchowicz, Przemysław Sosnowski, Edmund Grześkowiak

Med Sci Monit 1998; 4(2): BR222-227 :: ID: 502544

Abstract

Many metabolic and morphological changes in tissues and others organs during the ischemic-reperfusion period are caused by free radicals produced in the ischemic area. All organisms are provided with antioxidative systems to protect themselves against the toxic effect of oxygen and its derivatives. The aim of our study was to determine the influence of exogenous antioxidants: mannitol and ascorbic acid as well as allopurinol - an inhibitor of xanthine oxidase, on the level of free radicals and antioxidative activity of enzymes in rat skeletal muscles during ischemia and reperfusion. The experiments were performed on male Wistar rats. Acute ischemia of the posterior limb was produced by placing a subcutaneous ligature on the thigh at the level of the inguinal ligament. At this stage of the experiment the animals were divided into 4 groups: A-the reperfusion group, B-group receiving allopurinol, C-group receiving mannitol, D-group receiving ascorbic acid. Samples of skeletal muscles were taken from the thigh before removing the ligature during the 3rd, 6th and 10th minute of reperfusion. We determined: the concentration of free oxygen radicals and the activity of superoxide dismutase, catalase and glutathione peroxidase. Treatment of rats subjected to ischemia-reperfusion with mannitol, allopurinol and ascorbic acid caused a decrease in the level of free radicals in ischemic skeletal muscles. Allopurinol proved to be an effective antioxidant which produced a statistically significant decrease in the level of free radicals. This may suggest that the inhibition of xanthine oxidase - a potential source of reactive oxygen forms after restoration of blood flow in acutely ischemic areas can reduce the effects of oxidative stress during the ischemic-reperfusion period.

Keywords: ischemia-reperfusion syndrome, Free Radicals, Xanthine Oxidase, Allopurinol, Ascorbic Acid, Mannitol

0 Comments

Editorial

01 December 2022 : Editorial  

Editorial: The World Health Organization (WHO) Fungal Priority Pathogens List in Response to Emerging Fungal Pathogens During the COVID-19 Pandemic

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.939088

Med Sci Monit 2022; 28:e939088

SARS-CoV-2/COVID-19

29 November 2022 : Clinical Research  

Retrospective Study to Identify Risk Factors for Severe Disease and Mortality Using the Modified Early Warn...

Med Sci Monit In Press; DOI: 10.12659/MSM.938647  

24 November 2022 : Clinical Research  

A Prospective Questionnaire-Based Study to Evaluate Factors Affecting the Decision to Receive COVID-19 Vacc...

Med Sci Monit In Press; DOI: 10.12659/MSM.938665  

01 November 2022 : Clinical Research  

Questionnaire-Based Study of 81 Patients in Poland to Evaluate the Course of Inflammatory Bowel Disease and...

Med Sci Monit 2022; 28:e938243

In Press

05 Dec 2022 : Database Analysis  

Impact of the De Ritis Ratio on the Prognosis of Patients with Stable Coronary Artery Disease Undergoing Pe...

Med Sci Monit In Press; DOI: 10.12659/MSM.937737  

02 Dec 2022 : Clinical Research  

Value of Early Laparoscopic Exploration for Primary Infertile Patients with Patent Fallopian Tubes Complica...

Med Sci Monit In Press; DOI: 10.12659/MSM.938637  

30 Nov 2022 : Clinical Research  

Retrospective Evaluation of Hematological and Clinical Factors Associated with 30-Day Mortality in 170 Pati...

Med Sci Monit In Press; DOI: 10.12659/MSM.938674  

29 Nov 2022 : Clinical Research  

Retrospective Study to Identify Risk Factors for Severe Disease and Mortality Using the Modified Early Warn...

Med Sci Monit In Press; DOI: 10.12659/MSM.938647  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

01 Nov 2020 : Review article  

Long-Term Respiratory and Neurological Sequelae of COVID-19

DOI :10.12659/MSM.928996

Med Sci Monit 2020; 26:e928996

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750