01 January 1998
Different intracellular calcium pools used by ethanol, vasopressin and bradykinin in perfused livers
Zoltan Ruttner, Laszlo Ligeti, Leslie Reinlib, Alan Charles McLaughlinMed Sci Monit 1998; 4(1): BR9-14 :: ID: 502803
Abstract
The effect of ethanol, vasopressin and bradykinin on cytosolic Ca2+ concentration ([Ca2+]i) levels was studied upon in the perfused livers obtained from Wistar rats. [Ca2+]i was measured using a fluorescence Ca2+ Indo-1 probe. The ED50 was found to be 500 mM for ethanol and 10 nM for vasopressin. Ethanol had a long lasting effect in elevating [Ca2+]i levels in contrast to the transient effect of vasopressin. Vasopressin failed to increase [Ca2+]i levels when applied after ethanol and vice versa. Depletion of intracellular Ca2+ stores by ryanodine and bradykinin (10 nM) had no effect on [Ca2+]i levels. In contrast, ethanol (500 mM) and vasopressin (10 nM) caused an increase in the calcium concentration level [Ca2+]i by 49% and 44% respectively, as compared to the control group. Perfusion with calcium free solution following ryanodine treatment resulted in an ineffectiveness of all three calcium agonists. This data suggest that ethanol and vasopressin increase the Ca2+ flux through the cell membrane and that they seem to influence the same intracellular calcium pools.
Keywords: Fluorometry, Ryanodine, Inositol, Rat
Editorial
01 March 2024 : Editorial
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-ThalassemiaDOI: 10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
In Press
21 Feb 2024 : Clinical Research
Potential Value of HSP90α in Prognosis of Triple-Negative Breast CancerMed Sci Monit In Press; DOI: 10.12659/MSM.943049
22 Feb 2024 : Review article
Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...Med Sci Monit In Press; DOI: 10.12659/MSM.943168
23 Feb 2024 : Clinical Research
A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...Med Sci Monit In Press; DOI: 10.12659/MSM.943732
26 Feb 2024 : Clinical Research
Predictive Value of Combined HbA1c and Neutrophil-to-Lymphocyte Ratio for Diabetic Peripheral Neuropathy in...Med Sci Monit In Press; DOI: 10.12659/MSM.942509
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952