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01 January 1998

Relationship between LPS E. coli and Intralipid in the influence of peripheral blood neutrophils examined by the NBT reduction tests

Ewa Pawłowska, Gabriel Turowski

Med Sci Monit 1998; 4(1): BR57-63 :: ID: 502827


The effect of LPS E. coli in different concentrations: 0.2, 1.0, 2.0, 4.0, 5.0 µg in constant volume of 0.01 ml of KRB and of Intralipid (ILP) in different dosages: 0.5, 1.0, 1.5, 2.0, 3.0 mg in 0.01 ml KRB, and also the mutual influence of LPS E. coli and Intralipid on metabolic activity of neutrophils reduction tests of sodium salt of nitroblue tetrazolium (NBT) were used.Stimulating effect of LPS E. coli was depended on used dosage. Dosages 0.2-1.0 µg of LPS proved to be insufficient for the stimulation of neutrophils. However statistically significant differences, in comparison to control, were ascertained for dosages 2.0-5.0 µg of LPS. Intralipid caused stimulation of neutrophils conditioned by size of used dosage. Obtained results of NBT test, stimulated by different ILP dosages, were highly statistically significant in comparison to control results. In combine use of ILP and LPS, for small dosages of LPS (o, 2-2.0 µg) stimulation was caused by ILP. In the use of 5.0 µg dosage of LPS statistically significant differences, in results of influence of LPS and ILP used together, in comparison to results of effect of LOPS, as well as ILP, when those factors worked separately were ascertained. It may point to stimulating effect of both factors combined on the metabolic activity of neutrophils.he research did not show a function of Intralipid in the neutralization of LPS E. coli. The experiment enable the choice of dosages: 4.0 µg LPS E. coli, and 2.0 mg of Intralipid as optimal for farther research of role of neutrophils in neutralization of endotoxin.

Keywords: Neutrophils, endotoxin (LPS), Intralipid (ILP), NBT reduction test



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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750