Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

30 April 1999

Influence of immunosupressive drugs on liver regeneration after partial hepatectomy in rats - experimental studies

Wojciech G. Polak

Med Sci Monit 1999; 5(3): BR405-409 :: ID: 504729


The aim of this study was to asses the influence of selected immunosuppressants on liver regeneration in rats. Material and methods: In sixty male Buffalo strain rats partial hepatectomy (60-65%) was carried out according to Higgins and Anderson under ether anaesthesia. The observation of the rats lasted 6 weeks and 42 days after operation all animals were sacrificed. Rats were randomly assigned to one of six group (10 rats per group) and treated as follows: group 1 - without immunosuppressant (control group), group 2 - cyclosporin A, group 3 - azathioprine, group 4 - tacrolimus, group 5 - prednisolone, group 6 - triple drug therapy (TDT) - azathioprine with cyclosporin A and prednisolone. All rats in each group had following laboratory investigations in blood serum: aspartate aminotransferase (AspAT) activity, alanine aminotransferase (AlAT) activity and alkaline phosphatase (AP) activity. The above parameters were assessed one day before operation and then in 21st and 42nd day after hepatectomy. After 42 days observation all rats were killed and the remnant liver were removed for investigation of hepatic restoration and histologic features. Histologic examination of all sections was performed in light microscope. Furthermore resected liver tissue was weighed the day of operation and also the remnant liver was weighed after sacrification of the animal to calculate the regenerative rate. When applicable the comparison for statistical significance was performed according to the Student's test, Scheffe's test and analysis of variance. A p value of<0.05 was considered to represent a significant difference. Result and conclusions. Regenerative rate of liver tissue was significantly higher in rats treated with cyclosporin A and tacrolimus and significantly lower in rats treated with prednisolone and TDT. There were no significant difference between control group and rats treated with azathioprine. All immunosupressants used in this study influenced on liver function parameters as AspAT, AlAT and AP activity, but the changes were temporary. Histologic examination revealed in 3 rats treated with cyclosporin A restoration nodules with hepatocytes regeneration. In two rats treated with azathioprine there was observed adenocarcinoma coming from bile ducts. One rat treated with tacrolimus presented acute, diffuse necrosis of central parts of hepatic lobules. This study has shown that among immunosupressant used in this experiment only cyclosporin A and tacrolimus stimulated liver regeneration after partial hepatectomy. On the other hand prednisolone and triple drug therapy inhibited liver regeneration, whereas azathioprine had no influence on liver restoration. In some rats treated with cyclosporin A restoration nodules with hepatocytes regeneration occured. In rats receiving azathioprine there were observed two cases of cholangiocarcinoma.

Keywords: Rat, histological examination, regenerative rate, regenerative rate, Tacrolimus, Steroids, Steroids, azathioprine



01 December 2022 : Editorial  

Editorial: The World Health Organization (WHO) Fungal Priority Pathogens List in Response to Emerging Fungal Pathogens During the COVID-19 Pandemic

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.939088

Med Sci Monit 2022; 28:e939088


29 November 2022 : Clinical Research  

Retrospective Study to Identify Risk Factors for Severe Disease and Mortality Using the Modified Early Warn...

Med Sci Monit In Press; DOI: 10.12659/MSM.938647  

24 November 2022 : Clinical Research  

A Prospective Questionnaire-Based Study to Evaluate Factors Affecting the Decision to Receive COVID-19 Vacc...

Med Sci Monit In Press; DOI: 10.12659/MSM.938665  

01 November 2022 : Clinical Research  

Questionnaire-Based Study of 81 Patients in Poland to Evaluate the Course of Inflammatory Bowel Disease and...

Med Sci Monit 2022; 28:e938243

In Press

05 Dec 2022 : Database Analysis  

Impact of the De Ritis Ratio on the Prognosis of Patients with Stable Coronary Artery Disease Undergoing Pe...

Med Sci Monit In Press; DOI: 10.12659/MSM.937737  

02 Dec 2022 : Clinical Research  

Value of Early Laparoscopic Exploration for Primary Infertile Patients with Patent Fallopian Tubes Complica...

Med Sci Monit In Press; DOI: 10.12659/MSM.938637  

30 Nov 2022 : Clinical Research  

Retrospective Evaluation of Hematological and Clinical Factors Associated with 30-Day Mortality in 170 Pati...

Med Sci Monit In Press; DOI: 10.12659/MSM.938674  

29 Nov 2022 : Clinical Research  

Retrospective Study to Identify Risk Factors for Severe Disease and Mortality Using the Modified Early Warn...

Med Sci Monit In Press; DOI: 10.12659/MSM.938647  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

01 Nov 2020 : Review article  

Long-Term Respiratory and Neurological Sequelae of COVID-19

DOI :10.12659/MSM.928996

Med Sci Monit 2020; 26:e928996

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750