Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

Selected markers of oxidative stress in rats with active Heymann nephritis

Agnieszka Korolczuk, Grażyna Wó—jcicka, Daniel Chibowski, Jerzy Bełtowski

Med Sci Monit 2001; 7(3): BR369-376 :: ID: 509897

Abstract

Background: Reactive oxygen species play an important role in the pathogenesis of different glomerulopathies. The purpose of this study was to examine selected markers of oxidative stress and antioxidant defense in active Heymann nephritis (AHN), which is a model of human membranous nephropathy.
Material/Methods: AHN was induced in female Wistar rats by i. p. injection of proximal tubule brush border antigen (Fx1A). The control animals received an equivolume injection of saline. The animals were sacrificed at 6, 9, 15, 17, 18, 19, 22 and 23 weeks after Fx1A administration. We assayed the plasma concentration of thiobarbituric acid-reactive substances (TBARS) and nitric oxide metabolites (nitrites + nitrates, NOx) as well as total plasma antioxidant capacity (ferric reducing ability of plasma, FRAP) and serum paraoxonase (PON) activity.
Results: Fx1A-injected rats demonstrated a marked increase in proteinuria and impairment of renal excretory function evidenced by increased plasma creatinine and uric acid. Histologically, diffuse renal changes were seen, characterized by glomerular hypercellularity, increased glomerular size and narrowing of Bowman's space. Ultrastructural studies revealed diffuse fusion of foot processes and detachment of podocytes, subepithelial immune deposits in the glomerular capillary wall, thickening of the glomerular basement membrane, and local accumulation of lymphocytes and macrophages in glomeruli and interstitial cells. In the AHN group, TBARS increased significantly beginning in the 9th week, reaching maximum at the 23rd week (139.4 % of time-matched control). The plasma concentration of NOx demonstrated a biphasic increase. The first peak (249.9 % of control) was observed at the 9th week, followed by a decrease to normal between the 17th and 19th week. Then the NOx concentration increased to 211% of control at the 23rd week. FRAP began to increase in the 9th week and reached its maximum (134.9 % of control) at the 15th week. After the 18th week FRAP returned progressively to control levels. PON activity was 17.0%, 19.1% and 21.3% lower in AHN than in the control group at the 19th, 22nd, and 23rd weeks, respectively.
Conclusions: These results indicate that AHN is associated with oxidant-antioxidant imbalance, which may contribute to renal damage in this model of glomerulonephritis.

Keywords: active Heymann nephritis, Nitric Oxide, Lipid Peroxidation, antioxidant capacity, paraoxonase

Add Comment 0 Comments

Editorial

01 March 2024 : Editorial  

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-Thalassemia

Dinah V. Parums

DOI: 10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

In Press

18 Mar 2024 : Clinical Research  

Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative Study

Med Sci Monit In Press; DOI: 10.12659/MSM.944136  

0:00

21 Feb 2024 : Clinical Research  

Potential Value of HSP90α in Prognosis of Triple-Negative Breast Cancer

Med Sci Monit In Press; DOI: 10.12659/MSM.943049  

22 Feb 2024 : Review article  

Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943168  

23 Feb 2024 : Clinical Research  

A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943732  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750