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31 October 2012

Proteome analysis of human cerebrospinal fluid as a diagnostic biomarker in patients with meningioma

Jae Ho KimBCDE, Sang Kwang LeeD, Yong Cheol YooB, Nam Hyun ParkB, Dan Bi ParkB, Jong Shin YooB, Hyun Joo AnB, Young Mok ParkAEG, Kyung Gi ChoAEG

DOI: 10.12659/MSM.883538

Med Sci Monit 2012; 18(11): BR450-460

Abstract

Background: To identify meningioma-specific proteins, cerebrospinal fluid (CSF) from 4 patients with a meningioma and 4 patients with a non-brain tumorous lesion were analyzed.
Material/Methods: Two-dimensional electrophoresis and electrospray quadrupole time-of-flight tandem mass spectrometry analyses revealed 10 unique spots, containing 11 independent proteins (spot #2 and #4 each contained 2 proteins and spot #3 was not identified) were evident in CSF associated with human meningioma: serum albumin precursor (3 different isoforms), Apolipoprotein E (Apo E), Apolipoprotein J precursor (Apo J), Transthyretin precursor (TTR), Prostaglandin D2 synthase 21kDa (PTGDS), proapolipoprotein, Chain D hemoglobin Ypsilanti, alpha-1-antitrypsin (AAT), and beta-2-microglobulin precursor (beta2M).
Results: The contents of Apo E, Apo J and AAT were increased, while PTGDS, TTR and beta2M were decreased.
Conclusions: The results observed by 2-dimensional electrophoresis were verified by Western blot analysis. The unique proteins may represent possible candidate biomarkers of meningioma.

Keywords: Molecular Sequence Data, Neoplasm Proteins - chemistry, Molecular Sequence Annotation, Meningioma - diagnosis, Meningeal Neoplasms - diagnosis, Electrophoresis, Gel, Two-Dimensional, Blotting, Western, Amino Acid Sequence, Proteome - metabolism, Proteomics - methods, Reproducibility of Results, Spectrometry, Mass, Electrospray Ionization, Tumor Markers, Biological - cerebrospinal fluid

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750