Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

31 December 1969

Serodiagnostic and immunoprophylactic potential of a 78kDa protein of Leishmania donovani of Indian origin.

Mandira Mukherjee, Anindita Bhattacharyya, Swadesh Duttagupta

Med Sci Monit 2002; 8(4): BR117-122 :: ID: 420881

Abstract

BACKGROUND: The serodiagnosis of visceral leishmaniasis due to Leishmaniadonovani using crude parasite antigen is complicated in many endemic areas by cross-reactions with serumfrom humans infected with other protozoan diseases. The search for pure antigens avoiding such cross-reactionsis in progress. Developing a vaccine against cutaneous leishmaniasis has been much more successful thanagainst visceral leishmaniasis. Immunoprophylactic studies using various combinations of antigens andadjuvants are also in progress, and several strategies are in use, with varying degrees of success. MATERIAL/METHODS:Promastigotes of Leishmania donovani were used. The 78kDa protein was purified by a monoclonal antibodytagged CNBr sepharose CL-4B column. The presence of the protein in both stages of the parasite and inkala-azar patient serum was analyzed by western blotting. ELISA was used for serodiagnosis and isotypeanalysis of antibodies produced in immunized mice. Immunoprophylactic studies were carried out basedon in vitro transformation of amastigotes to promastigotes. RESULTS: The 78 kDa membrane protein, presentin both amastigote and promastigote forms of the parasite, was purified to homogeneity. The protein wasfound to have serodiagnostic potential to detect kala-azar. BALB/c mice immunized with 78kDa proteinrevealed reduction in spleen parasitemia. Isotype profiles of antibodies produced by immunized mice showedincreased production of IgG2a and decreased IgG1 levels. CONCLUSIONS: Our results demonstrated, for thefirst time, the serodiagnostic and immunoprophylactic use of a pure membrane protein isolated from Leishmaniadonovani of Indian origin.

Keywords: Antibodies, Monoclonal, Antibodies, Protozoan, Antigens, Protozoan, Immunoglobulin G, Immunoglobulin Isotypes, Immunoglobulin M, India, Leishmania donovani, Leishmaniasis, Visceral, Membrane Proteins, Mice, Inbred BALB C, Molecular Weight, Parasitemia, Protozoan Proteins, Protozoan Vaccines, Vaccination

0 Comments
Error 404
Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

Page Not Found

The page you are looking for was not found. Please use the search engine or the website menu.

Latest Articles

#937557

19 Aug 2022 : Database Analysis  

Use and Effects of Augmentation of Labor with Oxytocin: A Single-Center, Retrospective, Case-Control Study of 4350 Women in Warsaw, Poland, 2015-2020

Grażyna Bączek, Sylwia Rychlewicz ORCID logo [...]

DOI: 10.12659/MSM.937557

Med Sci Monit 2022; 28:e937557

19 Aug 2022 : Database Analysis  

Use and Effects of Augmentation of Labor with Oxytocin: A Single-Center, Retrospective, Case-Control Study of 4350 Women in Warsaw, Poland, 2015-2020

Grażyna Bączek, Sylwia Rychlewicz ORCID logo [...]

Department of Obstetrics and Gynecology Didactics, Medical University of Warsaw, Warsaw, Poland

DOI: 10.12659/MSM.937557

Med Sci Monit 2022; 28:e937557

#937331

18 Aug 2022 : Clinical Research  

A 3-Year Clinical Evaluation of Endodontically Treated Posterior Teeth Restored with Resin Nanoceramic Computer-Aided Design/Computer-Aided Manufacture (CAD/CAM)-Fabricated Partial Crowns

Lei Zhang, Xiao-Xia Hou [...]

DOI: 10.12659/MSM.937331

Med Sci Monit 2022; 28:e937331

#937331

18 Aug 2022 : Clinical Research  

A 3-Year Clinical Evaluation of Endodontically Treated Posterior Teeth Restored with Resin Nanoceramic Computer-Aided Design/Computer-Aided Manufacture (CAD/CAM)-Fabricated Partial Crowns

Lei Zhang, Xiao-Xia Hou [...]

DOI: 10.12659/MSM.937331

Med Sci Monit 2022; 28:e937331

#937146

16 Aug 2022 : Clinical Research  

Hemoglobin/Red Blood Cell Distribution Width Ratio in Peripheral Blood Is Positively Associated with Prognosis of Patients with Primary Hepatocellular Carcinoma

Yue Fang, Xiaonan Sun [...]

DOI: 10.12659/MSM.937146

Med Sci Monit 2022; 28:e937146

#937146

16 Aug 2022 : Clinical Research  

Hemoglobin/Red Blood Cell Distribution Width Ratio in Peripheral Blood Is Positively Associated with Prognosis of Patients with Primary Hepatocellular Carcinoma

Yue Fang, Xiaonan Sun [...]

DOI: 10.12659/MSM.937146

Med Sci Monit 2022; 28:e937146

#936569

15 Aug 2022 : Clinical Research  

Radiological Assessment of Prevalance and Quality of Periapical Status of Endodontic Treatments

Zeynep Aysal ORCID logo, Husniye Demirturk Kocasarac ORCID logo [...]

DOI: 10.12659/MSM.936569

Med Sci Monit 2022; 28:e936569

#936569

15 Aug 2022 : Clinical Research  

Radiological Assessment of Prevalance and Quality of Periapical Status of Endodontic Treatments

Zeynep Aysal ORCID logo, Husniye Demirturk Kocasarac ORCID logo [...]

DOI: 10.12659/MSM.936569

Med Sci Monit 2022; 28:e936569

#937041

12 Aug 2022 : Clinical Research  

Clinical Value of Metagenomic Next-Generation Sequencing in Immunocompromised Patients with Sepsis

Zheng Cheng, Feng Yu

DOI: 10.12659/MSM.937041

Med Sci Monit 2022; 28:e937041

#937041

12 Aug 2022 : Clinical Research  

Clinical Value of Metagenomic Next-Generation Sequencing in Immunocompromised Patients with Sepsis

Zheng Cheng, Feng Yu

DOI: 10.12659/MSM.937041

Med Sci Monit 2022; 28:e937041

#936637

11 Aug 2022 : Clinical Research  

Surgical Management of 48 Patients with Retrosternal Goiter and Tracheal Stenosis: A Retrospective Clinical Study from a Single Surgical Center

Tao Zuo, Zhaoming Gao [...]

DOI: 10.12659/MSM.936637

Med Sci Monit 2022; 28:e936637

#936637

11 Aug 2022 : Clinical Research  

Surgical Management of 48 Patients with Retrosternal Goiter and Tracheal Stenosis: A Retrospective Clinical Study from a Single Surgical Center

Tao Zuo, Zhaoming Gao [...]

DOI: 10.12659/MSM.936637

Med Sci Monit 2022; 28:e936637

#936745

10 Aug 2022 : Clinical Research  

A Retrospective Study from a Single Center to Identify Hematological Factors that Distinguish Between Patients with Colorectal Carcinoma and Colorectal Adenoma

Jun Huang, Pingping Xu [...]

DOI: 10.12659/MSM.936745

Med Sci Monit 2022; 28:e936745

#936745

10 Aug 2022 : Clinical Research  

A Retrospective Study from a Single Center to Identify Hematological Factors that Distinguish Between Patients with Colorectal Carcinoma and Colorectal Adenoma

Jun Huang, Pingping Xu [...]

DOI: 10.12659/MSM.936745

Med Sci Monit 2022; 28:e936745

#937338

09 Aug 2022 : Animal Research  

Label-Free Mass Spectrometry-Based Quantitative Proteomics to Evaluate the Effects of the Calcium-Sensing Receptor Agonist Cinacalcet on Protein Expression in Rat Brains and Livers

Ewa Bulska ORCID logo, Andrzej Gawor ORCID logo [...]

DOI: 10.12659/MSM.937338

Med Sci Monit 2022; 28:e937338

#937338

09 Aug 2022 : Animal Research  

Label-Free Mass Spectrometry-Based Quantitative Proteomics to Evaluate the Effects of the Calcium-Sensing Receptor Agonist Cinacalcet on Protein Expression in Rat Brains and Livers

Ewa Bulska ORCID logo, Andrzej Gawor ORCID logo [...]

DOI: 10.12659/MSM.937338

Med Sci Monit 2022; 28:e937338

#937528

08 Aug 2022 : Clinical Research  

Identification of Hepatic Dendritic Cells in Liver Biopsies Showing Steatosis in Patients with Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Associated with Obesity

Beatriz Barranco-Fragoso, Shreya C. Pal ORCID logo [...]

DOI: 10.12659/MSM.937528

Med Sci Monit 2022; 28:e937528

#937528

08 Aug 2022 : Clinical Research  

Identification of Hepatic Dendritic Cells in Liver Biopsies Showing Steatosis in Patients with Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Associated with Obesity

Beatriz Barranco-Fragoso, Shreya C. Pal ORCID logo [...]

DOI: 10.12659/MSM.937528

Med Sci Monit 2022; 28:e937528

#937630

05 Aug 2022 : Clinical Research  

Safety and Efficacy of the Baska Mask Versus Endotracheal Intubation in 64 Women Undergoing Elective Laparoscopic Gynecological Surgery Under General Anesthesia: A Prospective Single-Center Study

Hyoeun Ahn ORCID logo, Sang-Kee Min [...]

DOI: 10.12659/MSM.937630

Med Sci Monit 2022; 28:e937630

#937630

05 Aug 2022 : Clinical Research  

Safety and Efficacy of the Baska Mask Versus Endotracheal Intubation in 64 Women Undergoing Elective Laparoscopic Gynecological Surgery Under General Anesthesia: A Prospective Single-Center Study

Hyoeun Ahn ORCID logo, Sang-Kee Min [...]

DOI: 10.12659/MSM.937630

Med Sci Monit 2022; 28:e937630

#936738

04 Aug 2022 : Clinical Research  

Hair Sample Analysis of Residents from Olsztyn, Northeastern Poland, to Evaluate Levels of Bisphenol S and Bisphenol A: A Pilot Study

Sławomir Gonkowski, Manolis Tzatzarakis [...]

DOI: 10.12659/MSM.936738

Med Sci Monit 2022; 28:e936738

#936738

04 Aug 2022 : Clinical Research  

Hair Sample Analysis of Residents from Olsztyn, Northeastern Poland, to Evaluate Levels of Bisphenol S and Bisphenol A: A Pilot Study

Sławomir Gonkowski, Manolis Tzatzarakis [...]

DOI: 10.12659/MSM.936738

Med Sci Monit 2022; 28:e936738

#936713

03 Aug 2022 : Clinical Research  

Evaluation of Bromelain-Based Enzymatic Debridement Combined with Laser Doppler Imaging and Healing of Burn Wounds

Tomasz Korzeniowski, Jerzy Strużyna [...]

DOI: 10.12659/MSM.936713

Med Sci Monit 2022; 28:e936713

#936713

03 Aug 2022 : Clinical Research  

Evaluation of Bromelain-Based Enzymatic Debridement Combined with Laser Doppler Imaging and Healing of Burn Wounds

Tomasz Korzeniowski, Jerzy Strużyna [...]

DOI: 10.12659/MSM.936713

Med Sci Monit 2022; 28:e936713

Editorial

15 August 2022 : Editorial  

Editorial: The Metabolic (Dysfunction) Associated Fatty Liver Disease (MAFLD)-Non-Alcoholic Fatty Liver Disease (NAFLD) Debate: A Forced Consensus and The Risk of a World Divide

Nahum Méndez-Sánchez, Ming-Hua Zheng, Takumi Kawaguchi, Shiv K. Sarin
Liver Research Unit, Medica Sur Clinic & Foundation and Faculty of Medicine, National Autonomous University of Mexico, Mexico City, Mexico

DOI: 10.12659/MSM.938080

Med Sci Monit 2022; 28:e938080

SARS-CoV-2/COVID-19

18 August 2022 : Database Analysis  

Transcriptome Analysis of Peripheral Blood Mononuclear Cells Response in Patients with Severe COVID-19 Reve...

Med Sci Monit In Press; DOI: 10.12659/MSM.937532  

In Press

19 Aug 2022 : Clinical Research  

Postoperative Outcomes Following a Modified Method of Surgical Division of the Splenic Pedicle in 719 Patie...

Med Sci Monit In Press; DOI: 10.12659/MSM.937763  

18 Aug 2022 : Database Analysis  

Transcriptome Analysis of Peripheral Blood Mononuclear Cells Response in Patients with Severe COVID-19 Reve...

Med Sci Monit In Press; DOI: 10.12659/MSM.937532  

18 Aug 2022 : Clinical Research  

Comparison of Local and Systemic Ibuprofen for Relief of Postoperative Pain in Symptomatic Teeth with Apica...

Med Sci Monit In Press; DOI: 10.12659/MSM.937339  

18 Aug 2022 : Clinical Research  

Cross-Cultural Adaptation of the Godin-Shephard Leisure-Time Physical Activity Questionnaire for Arabic Pop...

Med Sci Monit In Press; DOI: 10.12659/MSM.937245  

Most Viewed Current Articles

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

01 Nov 2020 : Review article  

Long-Term Respiratory and Neurological Sequelae of COVID-19

DOI :10.12659/MSM.928996

Med Sci Monit 2020; 26:e928996

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750

Editorial

01 December 2022 : Editorial  

Editorial: The World Health Organization (WHO) Fungal Priority Pathogens List in Response to Emerging Fungal Pathogens During the COVID-19 Pandemic

Dinah V. Parums
Science Editor, Medical Science Monitor, International Scientific Information, Inc., Melville, NY, USA

DOI: 10.12659/MSM.939088

Med Sci Monit 2022; 28:e939088

SARS-CoV-2/COVID-19

29 November 2022 : Clinical Research  

Retrospective Study to Identify Risk Factors for Severe Disease and Mortality Using the Modified Early Warn...

Med Sci Monit In Press; DOI: 10.12659/MSM.938647  

24 November 2022 : Clinical Research  

A Prospective Questionnaire-Based Study to Evaluate Factors Affecting the Decision to Receive COVID-19 Vacc...

Med Sci Monit In Press; DOI: 10.12659/MSM.938665  

01 November 2022 : Clinical Research  

Questionnaire-Based Study of 81 Patients in Poland to Evaluate the Course of Inflammatory Bowel Disease and...

Med Sci Monit 2022; 28:e938243

In Press

30 Nov 2022 : Clinical Research  

Retrospective Evaluation of Hematological and Clinical Factors Associated with 30-Day Mortality in 170 Pati...

Med Sci Monit In Press; DOI: 10.12659/MSM.938674  

29 Nov 2022 : Clinical Research  

Retrospective Study to Identify Risk Factors for Severe Disease and Mortality Using the Modified Early Warn...

Med Sci Monit In Press; DOI: 10.12659/MSM.938647  

28 Nov 2022 : Review article  

A Review of the Roles of Apelin and ELABELA Peptide Ligands in Cardiovascular Disease, Including Heart Fail...

Med Sci Monit In Press; DOI: 10.12659/MSM.938112  

25 Nov 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

Med Sci Monit In Press; DOI: 10.12659/MSM.937990  

Most Viewed Current Articles

13 Nov 2021 : Clinical Research  

Acceptance of COVID-19 Vaccination and Its Associated Factors Among Cancer Patients Attending the Oncology ...

DOI :10.12659/MSM.932788

Med Sci Monit 2021; 27:e932788

30 Dec 2021 : Clinical Research  

Retrospective Study of Outcomes and Hospitalization Rates of Patients in Italy with a Confirmed Diagnosis o...

DOI :10.12659/MSM.935379

Med Sci Monit 2021; 27:e935379

01 Nov 2020 : Review article  

Long-Term Respiratory and Neurological Sequelae of COVID-19

DOI :10.12659/MSM.928996

Med Sci Monit 2020; 26:e928996

08 Mar 2022 : Review article  

A Review of the Potential Roles of Antioxidant and Anti-Inflammatory Pharmacological Approaches for the Man...

DOI :10.12659/MSM.936292

Med Sci Monit 2022; 28:e936292

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750